4th WCSET-2015 at Japan

Plenary Session:

Title:           Multidisciplinary Integration Focus on Point-of-Care Testing
Authors:      Cheng-Hsin CHUANG
Abstract:     Point-of-care testing (POCT), or bedside testing is defined as medical testing at or near the site of patient care. In order to approach POCT, a miniaturization system consists of microfluidics chip, bio-recognition probes and electrically sensing element for providing enough information on clinical diagnosis. As the result, multidisciplinary researches and integrations are crucial for developing POCT. This work presents a modulelevel impedance measurement system integrated with a dielectrophoretic (DEP) biochip for the immunoassay of bladder cancer cell lysate (T24) to a specific antibody (Galectin-1). In this talk, we will introduce how to fabricate a multiplexed antibody array by multi-step dielectrophoretic manipulations of nanoparticles and the advantages of electrically sensing instead of conventional fluorescence signal, which requires bulky measurement instrument. Finally, we will demonstrate a low-cost and high sensitivity immunoassay can be completed in 10 min using a portable impedance system module. This immunoassay platform technology gives great potential for use in point-of-care diagnostics.

Keywords:   Immunosensor, Dielectrophoresis, Point of Care, Interdigital Microelectrode Array
Pages:         003-003

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