3th WCSET-2014 at Nepal
Special Session at Ho Chi Minh City University of Technology, Vietnam:
Title:
Screening for Immunomodulatory activity of fermented
Ophiocordyceps Sinensis
Authors:
Minh-Hiep Dinh, The-Phuong Nguyen, Thu Huynh,
Binh-Nguyen Truong
Abstract:
Ophiocordyceps sinensis, a well-known Chinese herbal
medicine, has been demonstrated to possess a myriad of
biological compounds and effects. In this study, we
evaluated the immunomodulatory activities of fermented
O. sinensis extracts on human peripheral blood
mononuclear cells (PBMC). The extracts were tested in
the PBMC collected from three healthy volunteers with
normal leukocyte cell index. At the concentrations of
50, 100 and 200 µg/ml, the BuOH and CPS significantly
increased the proliferation of PBMC (%) to 84.55 ±
21.85, 120.35 ± 43.07, 21.98 ± 9.75 and 20.14 ± 8.15,
16.69 ± 5.89, and 21.25 ± 11.75, respectively (p<0.05).
Furthermore, water extract at 100 µg/ml showed a
proliferation of was 16.99 ± 16.87 % (p<0.05). The
result indicated that the BuOH at 100 µg/ml exhibited
the highest proliferation rate. Besides, EtOH (only at
the concentration of 200 µg/ml), PE and EtOAc also
exhibited the inhibitory effects on the human PBMC. In
conclusion, these data revealed the immuno-enhancing
ability of O. sinensis, suggesting that further studies
on in vivo models would be necessary.
Keywords: Extract,
Immunomodulatory Activity, PBMC, Ophiocordyceps Sinensis
Pages:
666-669